MTD-like motif of a BH3-only protein, BNIP1, induces necrosis accompanied by an intracellular calcium spike

作者：Park Junghee; Han Ji Hye; Myung Seung Hyun; Seo Young Woo^*; Kim Tae Hyoung^*

来源：Biochemical and Biophysical Research Communications, 2018, 495(2): 1661-1667.

DOI：10.1016/j.bbrc.2017.12.022

• B1MLM
• BNIP1
• MTD
• NOXA
• Necrosis

The mitochondrial targeting domain (MTD) of Noxa has necrosis-inducing activity when conjugated with cell-penetrating peptide (CPP). In this study, we report another MTD-like motif, B1MLM, found in BNIP1, a pro-apoptotic BH3-only protein found in the endoplasmic reticulum membrane. The B1MLM peptide, conjugated with CPP, induced necrosis in a way similar to that of R8:MTD. R8:B1MLM caused an intracellular calcium spike, mitochondrial reactive oxygen species generation, and mitochondrial fragmentation. The cytosolic calcium spike was likely due to the opening of the mitochondrial permeability transition pore.

• 出版日期2018-1-8