Mapping owl%26apos;s eye cells of patients with cytomegalovirus corneal endotheliitis using in vivo laser confocal microscopy

作者：Yokogawa Hideaki; Kobayashi Akira^*; Sugiyama Kazuhisa

来源：Japanese Journal of Ophthalmology, 2013, 57(1): 80-84.

DOI：10.1007/s10384-012-0189-5

• Cytomegalovirus corneal endotheliitis
• Confocal microscopy
• Owl%26apos
• s eye cell
• Coin-shaped lesion

To produce a two-dimensional reconstruction map of owl%26apos;s eye cells using in vivo laser confocal microscopy in patients with cytomegalovirus (CMV) corneal endotheliitis, and to demonstrate any association between owl%26apos;s eye cells and coin-shaped lesions observed with slit-lamp biomicroscopy. %26lt;br%26gt;Two patients (75- and 77-year-old men) with polymerase chain reaction-proven CMV corneal endotheliitis were evaluated in this study. Slit-lamp biomicroscopy and in vivo laser confocal microscopy were performed. Images of owl%26apos;s eye cells in the endothelial cell layer were arranged and mapped into subconfluent montages. Montage images of owl%26apos;s eye cells were then superimposed on a slit-lamp photo of the corresponding coin-shaped lesion. Degree of concordance between the confocal microscopic images and slit-lamp photos was evaluated. %26lt;br%26gt;In both eyes, a two-dimensional reconstruction map of the owl%26apos;s eye cells was created by computer software using acquired confocal images; the maps showed circular patterns. Superimposing montage images of owl%26apos;s eye cells onto the photos of a coin-shaped lesion showed good concordance in the two eyes. %26lt;br%26gt;This study suggests that there is an association between owl%26apos;s eye cells observed by confocal microscopy and coin-shaped lesions observed by slit-lamp biomicroscopy in patients with CMV corneal endotheliitis. The use of in vivo laser confocal microscopy may provide clues as to the underlying causes of CMV corneal endotheliitis.

• 出版日期2013-1