An optimised protocol to prepare Phaeocystis globosa morphotypes for scanning electron microscopy observation

作者：Guiselin Natacha^*; Courcot Lucie; Artigas Luis Felipe; Le Jeloux Aude; Brylinski Jean Michel

来源：Journal of Microbiological Methods, 2009, 77(1): 119-123.

DOI：10.1016/j.mimet.2009.01.002

• Phaeocystis globosa morphotypes
• SEM preparation method
• Air-drying
• Phytoplankton fixatives and preservatives
• Intra colonial cells

A preparation protocol for scanning electron microscopy (SEM) was adapted and tested to the observation of Phaeocystis globosa morphotypes. For extra colonial cells, critical point drying (CPD) gave satisfactory results while for intra colonial cells, preservation with Lugol's iodine and/or glutaraldehyde followed by air-drying appeared as the most suitable method.

• 出版日期2009-4