Adherence of human mesenchymal stem cells on Ti and TiO2 nano-columnar surfaces fabricated by glancing angle sputter deposition

作者：Motemani Yahya^*; Greulich Christina; Khare Chinmay; Lopian Michael; Buenconsejo Pio John S; Schildhauer Thomas A; Ludwig Alfred; Koeller Manfred

来源：Applied Surface Science, 2014, 292: 626-631.

DOI：10.1016/j.apsusc.2013.12.022

• Human mesenchymal stem cells
• Cell adhesion
• Nanostructure
• Titanium
• Glancing angle sputter deposition

The interaction of human mesenchymal stem cells (hMSCs) with Ti and TiO2 nano-columnar surfaces fabricated using glancing angle sputter deposition was investigated. The adherence and proliferation of hMSCs on different nano-columnar surfaces, including vertical columns, slanted columns and chevrons, were examined with calcein-acetoxymethyl ester fluorescence staining and scanning electron microscopy. For comparison, adherence of hMSCs on compact, dense films was also studied. After 24 hand 7 days, adherent and viable cells were observed on both, Ti nano-columns as well as dense Ti films, which confirms the biocompatibility of these nanostructures. Very small pseudopodia with width of approximately 20-35 nm and length varying from 20 to 200 nm were observed between the nano-columns, independent of the type of the nano-columnar morphology. Large inter-column spacing and effectively increased surface area make these nanostructures promising candidates for bio-functionalization or drug loading on the surface of Ti-based implants.

• 出版日期2014-2-15