A COLD-SENSITIVE MESSENGER-RNA SPLICING MUTANT IS A MEMBER OF THE RNA HELICASE GENE FAMILY

作者：STRAUSS EJ^*; GUTHRIE C

来源：Genes & Development, 1991, 5(4): 629-641.

DOI：10.1101/gad.5.4.629

• SPLICING
• HELICASE
• SACCHAROMYCES-CEREVISIAE

We have isolated a cold-sensitive mutant of Saccharomyces cerevisiae in which the first step of mRNA splicing is inhibited. The growth and splicing defects are recessive and cosegregate, thus defining a single essential gene (PRP28). The wild-type PRP28 gene was cloned, and sequence analysis reveals extensive homology to a family of proteins that are thought to function as ATP-dependent RNA helicases. The cold sensitivity is caused by a glycine-to-glutamic acid change in a conserved sequence motif. Interestingly, double mutants containing conditional alleles of PRP28 and PRP24, which encodes a U6 snRNA-binding protein, are inviable. In addition, a suppressor of prp28-1 is a mutant allele of PRP8, which encodes a U5 protein, thus linking PRP28 with U5. These data are consistent with a scenario in which PRP28 acts to unwind the U4/U6 base-pairing interaction in the U4/U6/U5 snRNP, facilitating the first covalent step of splicing.

• 出版日期1991-4