摘要
Fluorescent lateral flow immunoassay (FLFIA) based on immunomagnetic separation (IMS) has the advantage of sensitivity. However, its complex operation includes IMS, elution, incubation, and FLFIA steps. Here, we prepared a core@shell@satellite structure fluorescent magnetic nanobeads (FMNBs) and firstly introduced them into the novel method that integrated IMS with FLFIA (I-IMS-FLFIA) for the detection of Escherichia coli O157:H7. The FMNBs exhibited excellent magnetic and fluorescent properties for applications in IMS and FLFIA. However, the inner filter effect (IFE) of FMNBs may disturb the detection of I-IMS-FLFIA. Systematical studies showed that the amount of immuno-FMNBs and the concentration of monoclonal antibody can be controlled to obtain maximum photoluminescence intensity and effectively weaken or solve IFE. Under optimum conditions, this method allows for the quantified detection of 2.39x10(2) CFU/mL and qualified detection of 2.50x10(3) CFU/mL. The method is simple, safe, efficient, and sensitive for the detection of foodborne pathogens.
- 出版日期2019-3-15
- 单位中国科学院; 食品科学与技术国家重点实验室; 南昌大学; 中国科学院宁波材料技术与工程研究所; 江西省林业科学院; 江西省疾病预防控制中心