Antibiotic resistance and molecular characterization of the hydrogen sulfide-negative phenotype among diverse Salmonella serovars in China

作者：Xie Jing; Wu Fuli; Xu Xuebin; Yang Xiaoxia; Zhao Rongtao; Ma Qiuxia; Li Peng; Wang Ligui; Hao Rongzhang; Jia Leiji; Du Xinying; Qiu Shaofu^*; Song Hongbin^*

来源：BMC Infectious Diseases, 2018, 18(1): 292.

DOI：10.1186/s12879-018-3209-3

• Multidrug resistance
• Hydrogen sulfide
• Salmonella
• Serogroup
• phs operon

Background: Among 2179 Salmonella isolates obtained during national surveillance for salmonellosis in China from 2005 to 2013, we identified 46 non-H2S-producing strains originating from different sources.
Methods: The isolates were characterized in terms of antibiotic resistance and genetic variability by pulsed-field gel electrophoresis and multilocus sequence typing. Mutation in the phs operon, which may account for the non-H2S-producing phenotype of the isolated Salmonella strains, was performed in this study.
Results: Among isolated non-H2S-producing Salmonella strains, more than 50% were recovered from diarrhea patients, of which H2S-negative S. Gallinarum, S. Typhimurium, S. Choleraesuis and S. Paratyphi A isolates constituted 76%. H2S-negative isolates exhibited a high rate of resistance to ticarcillin, ampicillin, and tetracycline, and eight of them had the multidrug resistance phenotype. Most H2S-negative Salmonella isolates had similar pulsed-field gel electrophoresis profiles and the same sequence type as H2S-positive strains, indicating a close origin, but carried mutations in the phsA gene, which may account for the non-H2S-producing phenotype.
Conclusions: Our data indicate that multiple H2S-negative strains have emerged and persist in China, emphasizing the necessity to implement efficient surveillance measures for controlling dissemination of these atypical Salmonella strains.

• 出版日期2018-7-3
• 单位中国人民解放军军事医学科学院; 上海市疾病预防控制中心