A general protein purification and immobilization method on controlled porosity glass: biocatalytic applications

作者:Cassimjee K Engelmark*; Kadow M; Wikmark Y; Humble M Svedendahl; Rothstein M L; Rothstein D M; Backvall J E
来源:Chemical communications, 2014, 50(65): 9134-9137.
DOI:10.1039/c4cc02605e

摘要

A general combined purification and immobilization method to facilitate biocatalytic process development is presented. The support material, EziG (TM), is based on controlled porosity glass (CPG) or polymer-coated versions thereof (HybCPG) and binds protein affinity tags. Biocatalytic reactions in aqueous and organic media with seven enzymes of biocatalytic interest are shown.

  • 出版日期2014-8-21