Alternative energy production pathways in Taenia crassiceps cysticerci in vitro exposed to a benzimidazole derivative (RCB20)

作者：Fraga Carolina Miguel; Da Costa Tatiane Luiza; De Castro Ana Maria; Reynoso Ducoing Olivia; Ambrosio Javier; Hernandez Campos Alicia; Castillo Rafael; Vinaud Marina Clare^*

来源：Parasitology, 2016, 143(4): 488-493.

DOI：10.1017/S0031182015001729

• Taenia crassiceps
• ketonic bodies
• fatty acids oxidation
• protein catabolism
• benzimidazole derivative

Biochemical studies of benzimidazole derivatives are important to determine their mode of action and activity against parasites. The lack of antihelminthic alternatives to treat parasitic infections and albendazole resistance cases make the search for new antiparasitary drugs of utmost importance. The 6-chloro-5-(1-naphthyloxy)-2-(trifluoromethyl)-1H-benzimidazole (RCB20) is a benzimidazole derivative with promising effect. This study evaluated the effect of different concentrations of RCB20 in the alternative energetic pathway of in vitro Taenia crassiceps cysticerci. The parasites were in vitro exposed to 6.5 and 13 mu M of RCB20 and albendazole sulfoxide (ABZSO). The quantification of acetate, acetoacetate, beta-hydroxybutyrate, fumarate and propionate was performed by high-performance liquid chromatography. The quantification of urea, creatinine and total proteins was performed by spectrophotometry. The increase in beta-hydroxybutyrate reflects the enhancement of the fatty acid oxidation in the treated groups. Volatile fatty acids secretion, acetate and propionate, was increased in the treated groups. The secretion mechanisms of the treated parasites were impaired due to organic acids increased concentrations in the cysticerci. It is possible to conclude that the metabolic effect on alternative energetic pathways is slightly increased in the parasites treated with RCB20 than the ones treated with ABZSO.

• 出版日期2016-4