Potassium ion-dependent trehalose phosphorylase from halophilic Bacillus selenitireducens MLS10

作者：Nihira Takanori; Saito Yuka; Chiku Kazuhiro; Kitaoka Motomitsu; Ohtsubo Ken' ichi; Nakai Hiroyuki^*

来源：FEBS LETTERS, 2013, 587(21): 3382-3386.

DOI：10.1016/j.febslet.2013.08.038

• Trehalose phosphorylase
• Potassium ion-dependent
• Glycoside hydrolase family 65
• Bacillus selenitireducens

We discovered a potassium ion-dependent trehalose phosphorylase (Bsel_1207) belonging to glycoside hydrolase family 65 from halophilic Bacillus selenitireducens MLS10. Under high potassium ion concentrations, the recombinant Bsel_1207 produced in Escherichia coli existed as an active dimeric form that catalyzed the reversible phosphorolysis of trehalose in a typical sequential bi bi mechanism releasing beta-D-glucose 1-phosphate and D-glucose. Decreasing potassium ion concentrations significantly reduced thermal and pH stabilities, leading to formation of inactive monomeric Bsel_1207. %26lt;br%26gt;Structured summary of protein interactions:

• 出版日期2013-11-1