科研之友
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Objectives: The purpose of the present study was to evaluate the prognostic value of miR-199b-5p in breast cancer patients, as well as explore its effects on breast cancer cells. @@@ Methods: The expression level of miR-199b-5p in breast cancer tissues and paired non-cancerous tissues were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Chi-square test was used to evaluate the relationship between miR-199b-5p expression and clinical parameters. Besides, overall survival analysis was carried out with Kaplan-Meier curve with log rank test, and the prognostic value of miR-199b-5p in breast cancer was evaluated by cox regression model. In addition, MTT and transwell assays in vitro were used to estimate the effects of miR-199b-5p on breast cancer cells. @@@ Results: MiR-199b-5p was down-regulated in breast cancer tissues, compared with adjacent normal tissues (P < 0.05). Moreover, its decreased level was significantly correlated with advanced TNM stage (P = 0.008) and positive lymph node metastasis (P = 0.013). Cell experiments suggested that miR-199b-5p could regulate proliferation and invasion of breast cancer cells. In addition, we found that breast cancer patients with low miR-199b-5p expression level had poorer overall survival than those with high level (log rank test, P = 0.021). MiR-199b-5p was an independent prognostic factor for breast cancer patients (HR = 2.318, 95% CI = 1.086-4.949, P = 0.030). @@@ Conclusion: Down-regulated miR-199b-5p in breast cancer patients is associated with malignant clinical characteristics. MiR-199b-5p may be a potential prognostic biomarker for breast cancer.
- 出版日期2016-12
- 单位武汉大学
