A monophasic solution for isolation of RNA devoid of polymerase chain reaction-detectable genomic DNA contamination

作者：Ghosh Ujjal; Bose Shankhamala; Ghosh Trinath; Bandyopadhyay Tapas Kumar; Basu Utpal^*

来源：Analytical Biochemistry, 2015, 477: 50-52.

DOI：10.1016/j.ab.2015.02.018

• RNA
• Monophasic solution
• PCR

Commercially available reagents and published protocols are widely used for RNA isolation. However, genomic DNA contamination in isolated RNA is a potential problem. Here we describe a simple, inexpensive method for eliminating genomic DNA contamination beyond the level of PCR-based detection through reduction of the guanidine thiocyanate concentration (1.5 M) in a single monophasic solution based on Chomczynski-Sacchi reagents. The new method can be used to isolate small and large RNA species of high quality and can be completed within an hour.

• 出版日期2015-5-15