A rapid and sensitive enzyme-linked immunosorbent assay (ELISA) method and validation for progestogen multi-residues in feed

作者：Peng C F; Chen Y W; Chen H Q; Xu Ch L^*; Jin Z Y

来源：Journal of Animal and Feed Sciences, 2008, 17(3): 434-441.

• progestogens
• multi-residues
• ELISA
• feed

Some progestogens as anabolic steroids can be illegally used for increasing the efficiency of animal food production. The aim of the study was to develop convenient screening method against progestogens in feed. A sensitive enzyme-linked immunosorbent assay (ELISA) method was developed and optimized. The ELISA method is class-specific for four relative progestogens and the IC(50) of the method was 3.0 mu g/l for MPA. A simple feed pre-treatment method was developed for analysis progestogen residues in feed. The limits of, detection (LOD) were 2.0 mu g/kg for MPA, 5.0 mu g/kg for CMA, 4.6 mu g/kg for MEGA and 3.9 mu g/kg for HPA in the feed. The recovery was between 72.1 and 95.4% and the coefficients of variation (CV) were 7.2 to 15.8%. The results have showed the simple sample pre-treatment method was suitable for progestogen multi-residues rapid analysis in feed.

• 出版日期2008
• 单位江南大学; 江苏省原子医学研究所