Lysophosphatidylglycerol stimulates chemotactic migration in human natural killer cells

作者：Jo Seong Ho; Kim Sang Doo; Kim Jung Mo; Lee Ha Young; Lee Sun Young; Shim Jae Woong; Yun Jeanho; Im Dong Soon; Bae Yoe Sik^*

来源：Biochemical and Biophysical Research Communications, 2008, 372(1): 147-151.

DOI：10.1016/j.bbrc.2008.05.004

• lysophosphatidylglycerol
• natural killer cells
• chemotaxis
• extracellular signal-regulated protein kinase

We observed that lysophosphatidylglycerol (LPG) stimulates chemotactic migration in human natural killer (NK) cells. The LPG-induced chemotactic migration of NK cells was completely inhibited by pertussis toxin (PTX). LPG also stimulated the extracellular signal-regulated kinase (ERK) and Akt activities in NK cells. LPG-stimulated ERK activity was inhibited by PTX, indicating the involvement of PTX-sensitive G-proteins. The preincubation of NK cells with an ERK inhibitor (PD98059) or phosphoinositide-3-kinase (PI3K) inhibitors (wortmannin and LY294002) completely inhibited LPG-induced chemotactic migration, suggesting the essential role of ERK and PI3K in the process. Moreover, LPG-induced chemotactic migration in NK cell was inhibited by Ki16425, an LPA(1/3) receptor-selective antagonist, suggesting the involvement of the Ki16425-sensitive G-protein coupled receptor (GPCR) in the process. Taken together, the results indicate that LPG stimulates chemotactic migration in NK cells through GPCR, suggesting a new function of LPG as a modulator of NK cell functioning.

• 出版日期2008-7-18