Apoptosis mechanisms of human gastric cancer cell line MKN-45 infected with human mutant p27

作者：Zhu, Jin-Shui^*; Wang, Long; Cheng, Guo-Qiang; Li, Qin; Zhu, Zu-Ming; Zhu, Li

来源：World Journal of Gastroenterology, 2005, 11(47): 7536-7540.

DOI：10.3748/wjg.v11.i47.7536

• Gastric cancer
• Human mutant p27
• Cell line MKN-45

AIM: To explore the inducing effect of human mutant p27 gene on the apoptosis of the human gastric cancer cell line MKN-45 and its associated mechanisms. @@@ METHODS: The recombinant adenovirus Ad-p27mt was constructed to infect the human gastric cancer cell line MKN-45. Using flow cytometry, TUNEL assay and DNA fragment analysis, we measured the apoptotic effect of Ad-p27mt on the human gastric cancer cells. @@@ RESULTS: Ad-p27mt was successfully constructed and the infection efficiency reached 100%. After 18 h of infection, we observed an apoptotic hypodiploid peak on the flow cytometer before G1-S and apoptotic characteristic bands in the DNA electrophoresis. The apoptotic rate detected by TUNEL method was significantly higher in the Ad-p27mt group ( 89.4+/-3.12%) compared to the control group (3.12+/-0.13%, P < 0.01). @@@ CONCLUSION: Human mutant p27 can induce apoptosis of the human gastric cancer cells in vitro.

• 出版日期2005-12-21
• 单位上海交通大学