Simultaneous absolute measures of glabrous skin hemodynamic and light-scattering change in response to formalin injection in rats

作者：He Ji Wei; Kashyap Dheerendra; Trevino Lara A; Liu Hanli^*; Peng Yuan Bo

来源：Neuroscience Letters, 2011, 492(1): 59-63.

DOI：10.1016/j.neulet.2011.01.057

• Inflammation
• Hemoglobin
• Hemoglobin oxygen saturation
• Blood flow
• Optical reflectance
• Light scattering

Subcutaneous injection of formalin is a well-known model to study the nature of inflammatory pain. One of the cardinal signs of inflammation is redness, as a result of increased blood perfusion. We used an optical technology, light reflectance spectroscopy, to noninvasively obtain absolute measures of cutaneous hemodynamic components, including the concentrations of oxy- ([HbO]), deoxy- ([Hb]), total-hemoglobin ([HbT]), oxygen saturation (SO(2)), and the reduced light-scattering coefficient (mu s'). The objective is to assess the effect of formalin-induced skin inflammation on the aforementioned parameters. Six rats were injected with formalin (50 mu l, 3%) into left hind paw under pentobarbital anesthesia. Our results indicate prolonged increases in [HbO], [HbT], and SO(2) post injection only in the ipsilateral side. No statistically significant changes in [Hb] and mu s' occurred in either side. The arterial blood influx tends to be the major attribute of local hyperemia during inflammation. Thereby, [HbO] appears to be superior to [Hb] in measuring inflammation. In conclusion, the needle-probe-based light reflectance can be a feasible means to obtaining absolute measures of skin hemodynamic and light-scattering parameters when studying inflammatory pain.

• 出版日期2011-3-29