PgTeL, the lectin found in Punica granatum juice, is an antifungal agent against Candida albicans and Candida krusei

作者：da Silva Pollyanna Michelle; de Moura Maiara Celine; Gomes Francis Soares; Trentin Danielle da Silva; Silva de Oliveira Ana Patricia; Vieira de Mello Gabriela Souto; da Rocha Pitta Maira Galdino; Barreto de Melo Rego Moacyr Jesus; Breitenbach Barroso Coelho Luana Cassandra; Macedo Alexandre Jose; Bressan Queiroz de Figueiredo Regina Celia; Guedes Paiva Patricia Maria; Napoleao Thiago Henrique^*

来源：International Journal of Biological Macromolecules, 2018, 108: 391-400.

DOI：10.1016/j.ijbiomac.2017.12.039

• Antifungal activity
• Biofilm
• Lectin
• Cell wall
• Pomegranate

The pomegranate (Punica granatum) sarcotesta contains a chitin-binding lectin (PgTeL) with antibacterial activity against human pathogenic species. In this work, the structural stability of PgTeL was evaluated by fluorimetric analysis and the lectin was evaluated for cytotoxicity to human peripheral blood mononuclear cells (PBMCs) and antifungal activity against Candida albicans and Candida krusei. PgTeL folding was impaired when lectin was incubated at pH >= 6.0. On the other hand, the lectin did not undergo unfolding even when heated at 100 degrees C. PgTeL (1, 10, and 100 mu g/mL) was not cytotoxic to PBMCs. Antifungal activity was detected for C. albicans (MIC: 25 mu g/mL; MFC: 5011 mu g/mL) and C. krusei (MIC and MFC of 12.5 mu g/mL). Treatment of yeast cells with PgTeL resulted in decrease of intracellular ATP content even at sub-inhibitory concentrations (1/2MIC and 1/4MIC) and induced lipid peroxidation. In addition, PgTeL damaged the integrity of fungal cell wall of both species, with more pronounced effects in C krusei. The lectin showed significant antibiofilm activity on C. albicans at sub-inhibitory concentrations (0.195 and 0.39 mu g/mL). In conclusion, PgTeL is an anti-Candida agent whose action mechanism involves oxidative stress, energetic collapse, damage to the cell wall and rupture of yeast cells.

• 出版日期2018-3