Toll-Like Receptors 2 and 4 in Pigs%26apos; Kidneys Early After Autotransplantation Procedure

作者：Caban A^*; Oczkowicz G; Budzinski G; Suszka Switek A; Dolinska B; Ostrozka Cieslik A; Wieczorek J; Ryszka F; Wiaderkiewicz R; Cierpka L

来源：Transplantation Proceedings, 2014, 46(8): 2545-2547.

DOI：10.1016/j.transproceed.2014.09.035

Objectives. The aim of this paper was to evaluate mRNA expression of Toll-like receptors 2 (TLR2) and 4 (TLR4) and the adaptor protein myeloid differentiation primary-response protein 88 (MyD88) in pigs%26apos; kidneys 14 and 30 days after autotransplantation. %26lt;br%26gt;Methods. The research was conducted on 12 animals that underwent left renal transplantation procedure with further standardized rinsing with Biolasol solution and 24 hours%26apos; storage in 4 C; subsequently the kidneys were implanted in the right retroperitoneal space after right-side nephrectomy. Six randomly chosen animals (group I) were under observation for 14 days, the other 6 (group II) for 30 days. After these observation periods, the animals were killed and 4-g samples were collected from the renal cortex and medulla. %26lt;br%26gt;Results. Expression of mRNA in homogenates of collected samples were determined with the use of reverse-transcription polymerase chain reaction analysis. Obtained results in both groups, presented in relation to GAPDH, were compared with the use of Mann-Whitney U test. Stable graft function was observed in all animals from the 2nd day after the procedure. TLR2 in group I reached the mean value of 3.64 and was statistically significantly higher than in group II (2.19). Inverse proportion was observed in case of mRNA for TLR4: group II presented 2 times higher value than group I (0.25 vs 0.11). Similarly, significant difference was observed in MyD88 (group I, 0.067; group II, 0.45). %26lt;br%26gt;Conclusions. At 14 days after autotransplantation of a pig kidney, mRNA expression for TLR2 is dominant; later, expression increases for TLR4 and MyD88.

• 出版日期2014-10