Delivery of surface-mediated non-viral gene nanoparticles from ultrathin layer-by-layer multilayers

作者：Wang XueFei; Ren KeFeng; Lin QuanKui; Ji Jian^*

来源：Science China Chemistry, 2010, 53(3): 508-513.

DOI：10.1007/s11426-010-0081-4

• DNA nanoparticles
• layer-by-layer self-assembly
• biodegradability
• in situ transfection
• poly(lactic acid) scaffold

An efficient and safe gene delivery system remains a challenge in the development of gene therapy. Polycation-based gene nanoparticles are a typical non-viral gene delivery system, which are able to transfect cells in vitro and in vivo. This paper reported a facile method for constructing biodegradable multilayers via layer-by-layer self-assembly, in which the polycation-based gene nanoparticles were loaded. Through this surface-mediated delivery system, adherent cells on the multilayer could be transfected in situ. Gene nanoparticles-loaded multilayers transfect cells with higher efficiency than naked DNA-loaded multilayers because of the complex configuration of the DNA. DNA nanoparticles/PGA multilayers constructed on the scaffold surface could also realize in situ transfection on the adherent cells. The well-structured, easy-processed multilayers may provide a novel approach to precisely controlled delivery of gene nanoparticles, which may have potential applications for gene therapy in tissue engineering and medical implants.

• 出版日期2010-3
• 单位浙江大学