Analysis, design and implementation of a novel scheme for in-vivo control of synthetic gene regulatory networks

作者：Menolascina Filippo; di Bernardo Mario^*; di Bernardo Diego

来源：Automatica, 2011, 47(6): 1265-1270.

DOI：10.1016/j.automatica.2011.01.073

• in vivo control
• Gene regulatory networks
• Synthetic biology
• Microfluidics

This paper is concerned with the design and implementation of a simple but effective switching control strategy to regulate the dynamics of synthetic gene networks. The testbed circuit is IRMA, a recently developed network in S. Cerevisiae which is proposed as a suitable benchmark problem for control design. The proof-of-concept of an implementation of the control strategy in vivo is presented which is based on the use of microfluidics devices and fluorescence microscopy. Preliminary experimental results are given showing the good matching between the model predictions and the experimental observations.

• 出版日期2011-6