A novel protein refolding method integrating ion exchange chromatography with artificial molecular chaperone

作者：Zhang Qin Ming; Wang Chao Zhan^*; Liu Jiang Feng; Wang Li Li

来源：Chinese Chemical Letters, 2008, 19(5): 595-598.

DOI：10.1016/j.cclet.2008.03.030

• artificial molecular chaperone
• ion exchange chromatography
• protein refolding
• lysozyme
• protein folding liquid chromatography

Artificial molecular chaperone (AMC) and ion exchange chromatography (IEC) were integrated, thus a new refolding method, artificial molecular chaperone-ion exchange chromatography (AMC-IEC) was developed. Compared with AMC and IEC, the activity recovery of lysozyme obtained by AMC-IEC was much higher in the investigated range of initial protein concentrations, and the results show that AMC-IEC is very efficient for protein refolding at high concentrations. When the initial concentration of lysozyme is 180 mg/mL, its activity recovery obtained by AMC-IEC is still as high as 76.6%, while the activity recoveries obtained by AMC and IEC are 45.6% and 42.4%, respectively.

• 出版日期2008-5
• 单位西北大学