Proteomic analysis of cellular signaling

作者：Chen WG; White FM^*

来源：Expert Review of Proteomics, 2004, 1(3): 343-354.

DOI：10.1586/14789450.1.3.343

• affinity chromatography
• electrospray ionization
• liquid chromatography
• mass spectrometry
• protein phosphorylation
• proteomics
• tandem mass spectrometry

Protein phosphorylation events are key regulators of cellular signaling processes. In the era of functional genomics, rational drug design programs demand large-scale high-throughput analysis of signal transduction cascades. Significant improvements in the area of mass spectrometry-based proteomics have provided exciting opportunities for rapid progress toward global protein phosphorylation analysis. This review summarizes several recent advances made in the field of phosphoproteomics with an emphasis placed on mass spectrometry instrumentation, enrichment methods and quantification strategies. In the near future, these technologies will provide a tool that can be used for quantitative investigation of signal transduction pathways to generate new insights into biologic systems.

• 出版日期2004-10