Alterations of the Transcriptome of Sulfolobus acidocaldarius by Exoribonuclease aCPSF2

作者：Maertens Birgit; Amman Fabian; Manoharadas Salim; Zeichen Lukas; Orell Alvaro; Albers Sonja Verena; Hofacker Ivo; Blaesi Udo

来源：PLos One, 2013, 8(10): e76569.

DOI：10.1371/journal.pone.0076569

Recent studies identified a 5%26apos; to 3%26apos; exoribonuclease termed Sso-RNase J in the crenarchaeon Sulfolobus solfataricus (Sso), which has been reclassified to the aCPSF2 (archaeal cleavage and polyadenylation specificity factor 2) group of beta-CASP proteins. In this study, the Sso-aCPSF2 orthologue of Sulfolobus acidocaldarius (Saci-aCPSF2) was functionally characterized. Like Sso-aCPSF2, Saci-aCPSF2 degrades RNA with 5%26apos; to 3%26apos; directionality in vitro. To address the biological significance of Saci-aCPSF2, a deletion mutant was constructed, and the influence of Saci-aCPSF2 on the transcriptome profile was assessed employing high throughput RNA sequencing. This analysis revealed 560 genes with differential transcript abundance, suggesting a considerable role of this enzyme in RNA metabolism. In addition, bioinformatic analyses revealed several transcripts that are preferentially degraded at the 5%26apos; end. This was exemplarily verified for two transcripts by Northern-blot analyses, showing for the first time that aCPSF2 proteins play a role in 5%26apos; to 3%26apos; directional mRNA decay in the crenarchaeal clade of Archaea.

• 出版日期2013-10-7
• 单位上海生物信息技术研究中心