A "click" chemistry constructed affinity system for 2-oxoglutaric acid receptors and binding proteins

作者：Wang Yang; Assaf Zeinab; Liu Xinjun; Ziarelli Fabio; Latifi Amel; Lamrabet Otmane; Quelever Gilles; Qu Fanqi; Zhang Cheng Cai; Peng Ling^*

来源：Organic and Biomolecular Chemistry, 2014, 12(33): 6470-6475.

DOI：10.1039/c4ob01005a

An ingenious and specific affinity resin designed to capture the 2-oxoglutaric acid (2-OG) binding proteins was constructed by appending a 2-OG tag to the solid resin via a Cu-catalyzed Huisgen "click" reaction. The so-obtained affinity resin was able to recognize, retain and separate the established 2-OG binding protein NtcA in both the pure form and crude cellular extract, thus constituting a valuable means of searching for novel 2-OG receptors with a view to exploring the signalling pathways of 2-OG, a key Krebs cycle intermediate with unprecedented signalling functions.

• 出版日期2014
• 单位武汉大学