A transcriptomic analysis of the effect of genistein on Sinorhizobium fredii HH103 reveals novel rhizobial genes putatively involved in symbiosis

作者：Perez Montano F; Jimenez Guerrero I; Acosta Jurado S; Navarro Gomez P; Ollero F J; Ruiz Sainz J E; Lopez Baena F J; Vinardell J M

来源：Scientific Reports, 2016, 6(1): 31592.

DOI：10.1038/srep31592

<jats:title>Abstract</jats:title><jats:p><jats:italic>Sinorhizobium fredii</jats:italic> HH103 is a rhizobial soybean symbiont that exhibits an extremely broad host-range. Flavonoids exuded by legume roots induce the expression of rhizobial symbiotic genes and activate the bacterial protein NodD, which binds to regulatory DNA sequences called <jats:italic>nod</jats:italic> boxes (NB). NB drive the expression of genes involved in the production of molecular signals (Nod factors) as well as the transcription of <jats:italic>ttsI</jats:italic>, whose encoded product binds to <jats:italic>tts</jats:italic> boxes (TB), inducing the secretion of proteins (effectors) through the type 3 secretion system (T3SS). In this work, a <jats:italic>S. fredii</jats:italic> HH103 global gene expression analysis in the presence of the flavonoid genistein was carried out, revealing a complex regulatory network. Three groups of genes differentially expressed were identified: i) genes controlled by NB, ii) genes regulated by TB and iii) genes not preceded by a NB or a TB. Interestingly, we have found differentially expressed genes not previously studied in rhizobia, being some of them not related to Nod factors or the T3SS. Future characterization of these putative symbiotic-related genes could shed light on the understanding of the complex molecular dialogue established between rhizobia and legumes.</jats:p>

• 出版日期2016-8-19