Association of alcohol dehydrogenase polymorphisms and life-style factors with excessive alcohol intake within the Spanish population (EPIC-Spain)

作者：Munoz Xavier; Amiano Pilar; Celorrio David; Dorronsoro Miren; Sanchez Maria Jose; Huerta Jose M; Barricarte Aurelio; Arriola Larraitz; Navarro Carmen; Molina Montes Esther; Dolores Chirlaque M; Ardanaz Eva; Rodriguez Laudina; Duell Eric J; Hijona Elizabeth; Herreros Villanueva Marta; Sala Nuria; Bujanda Luis^*

来源：Addiction, 2012, 107(12): 2117-2127.

DOI：10.1111/j.1360-0443.2012.03970.x

• ADH6
• ADH1B
• excessive alcohol intake
• genetic susceptibility
• life-style factors
• polymorphisms

Aims To analyse associations between alcohol dehydrogenase (ADH) polymorphisms and alcohol intake in Spanish men and women. Design and settings We analysed the relationship between 21 genetic variants in ADH genes and excessive alcohol intake in both men and women. Single nucleotide polymorphisms (SNPs) were genotyped using a customized array and a sex-stratified analysis was performed. Measurements Ethanol intake was calculated using a validated dietary history questionnaire. Participants Heavy consumers of alcohol (=70 g/day in men, =42 g/day in women) (653 cases) and very low or non-consumers (%26lt;2 g/day) (880 controls) from the Spanish cohort of the European Prospective Investigation into Cancer (EPIC). Findings We found statistically significant associations between alcohol intake and known life-style factors; namely, smoking and food energy intake (meat and fruit/seeds) in both men and women, as well as with physical activity in women and educational level in men. Additionally, we found that a non-synonymous coding SNP in ADH1B (rs1229984) is associated inversely with excessive alcohol intake in men [odds ratio (OR) = 0.19, 95% confidence interval (CI) = 0.110.33; P = 4.77E(-10)) and women (OR = 0.48, 95% CI = 0.270.83; P = 0.0067). Furthermore, ADH6 rs3857224 was found associated with heavy alcohol intake in women (OR = 1.61, 95% CI = 1.212.14; P = 1.01E(-3)), but not in men. Conclusions In the Spanish population, the single nucleotide polymorphism of alcohol dehydrogenase ADH1B, rs1229984, is associated inversely with alcohol intake in both men and women. Another polymorphism of ADH6, rs3857224, is associated with heavy alcohol intake in women.

• 出版日期2012-12