Differential effects of chemoattractants on mast cell recruitment in vivo

作者：de Cassia Campos Maria Rita; Toso Vanina Danuza; de Souza Devandir Antonio Jr; Vieira Gabriel Viliod; Marcelino da Silva Elaine Zayas; Oliver Constance; Jamur Maria Celia^*

来源：Cellular Immunology, 2014, 289(1-2): 86-90.

DOI：10.1016/j.cellimm.2014.03.013

• Mast cells
• Recruitment
• IL-3
• SCF
• RANTES
• LTB4
• In vivo

Rats were injected with rat recombinant (rr) IL3, rrSCF, rrIL-3 + rrSCF, rrRANTES and LTB4. Six hours after subcutaneous injection of rrIL-3 or rrIL-3 + rrSCF, there was an increase in mast cell numbers in the skin and spleen. Peritoneal mast cells were recruited following i.p. injection of rrIL-3, but with rrIL-3 + rrSCF recruitment was delayed. Immunostaining with a mast cell specific antibody showed that immature orthochromatic mast cells were being recruited. rrIL-3 induced recruitment of mast cells to the peritoneal cavity was blocked by anti-integrin antibodies. Mast cell recruitment depended on the target tissue and the time of exposure to the chemoattractant.

• 出版日期2014-6