An LC-MS/MS method for quantitation of cyanidin-3-O-glucoside in rat plasma: Application to a comparative pharmacokinetic study in normal and streptozotocin-induced diabetic rats

作者：Yang, Chunxia; Wang, Qiuhua; Yang, Shenbao; Yang, Qiong^*; Wei, Ying

来源：Biomedical Chromatography, 2018, 32(2): e4042.

DOI：10.1002/bmc.4042

• cyanidin-3-O-glucoside
• diabetic rats
• LC-MS/MS
• pharmacokinetics

A sensitive and reliable liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed to determine cyanidin-3-O-glucoside (Cy-3G) in normal and streptozotocin-induced diabetic rat plasma. Chromatographic separation was carried out on a Zorbax SB-C-18 (50 x 4.6 mm, 5 mu m) column and mass spectrometric analysis was performed using a Thermo Finnigan TSQ Quantum Ultra triple-quadrupole mass spectrometer coupled with an ESI source in the negative ion mode. Selected reaction monitoring mode was applied for quantification using target fragment ions m/z 447.3 -> 285.2 for Cy-3G and m/z 463.0 -> 300.1 for quercetin-3-O-glucoside (internal standard). The calibration curve was linear over the range 3.00-2700 ng/mL (r(2) >= 0.99) with the lower limit of quantitation at 3.00 ng/mL. Intra- and inter-day precision was < 14.5% and mean accuracy was from -11.5 to 13.6%. Stability testing showed that Cy-3G remained stable during the whole analytical procedure. After validation, the assay was successfully used to support a preclinical pharmacokinetic comparison of Cy-3G between normal and diabetic rats. Results indicated that diabetes mellitus significantly altered the in vivo pharmacokinetic characteristics of Cy-3G after oral administration in rats.

• 出版日期2018-2
• 单位北京中医药大学