An extracellular solvent stable alkaline lipase from Pseudomonas sp DMVR46: Partial purification, characterization and application in non-aqueous environment

作者：Patel Vrutika; Nambiar Shruti; Madamwar Datta^*

来源：Process Biochemistry, 2014, 49(10): 1673-1681.

DOI：10.1016/j.procbio.2014.06.007

• Organic solvent stable lipase
• Pseudomonas sp.
• Purification
• Microemulsion based organogels (MBGs)
• Ester synthesis

The biosynthesis of esters is currently of much commercial interest because of the increasing popularity and demand for natural products among consumers. Biotransformation and enzymatic methods of ester synthesis are more effective when performed in non-aqueous media. In present study, an organic solvent stable Pseudomonas sp. DMVR46 lipase was partially purified by acetone precipitation and ion exchange chromatography with 28.95-fold purification. The molecular mass of the lipase was found to be similar to 32 kDa. The partially purified lipase was optimally active at 37 degrees C and pH 8.5. The enzyme showed greater stability toward organic solvents such as isooctane, cyclohexane and n-hexane retaining more than 70% of its initial activity. The metal ions such as Ca2+, Ba2+ and Mg2+ had stimulatory effects on lipase activity, whereas Co2+ and Zn2+ strongly inhibited the activity. Also lipase exhibited variable specificity/hydrolytic activity toward different 4-nitrophenyl esters. DMVR46 lipase was further immobilized into AOT-based organogels used for the synthesis of flavor ester pentyl valerate in presence of organic solvents. The organogels showed repeated use of enzyme with meager loss of activity even upto 10 cycles. The solvent-stable lipase DMVR46 thus proved to be an efficient catalyst showing an attractive potency for application in biocatalysis under non-aqueous environment.

• 出版日期2014-10