Diurnal rhythms of blood glucose, serum ghrelin, faecal IgA and faecal corticosterone in rats subjected to restricted feeding using the diet board

作者：Kasanen Iiris; Inhila Katja; Savontaus Eriika; Voipio Hanna Marja; Okva Kai; Mering Satu; Kiviniemi Vesa; Hau Jann; Laaksonen Sakari; Nevalainen Timo^*

来源：Laboratory Animals, 2018, 52(1): 29-37.

DOI：10.1177/0023677217709850

• rats
• restricted feeding
• diurnal rhythms
• refinement
• reduction

Laboratory rats are generally fed ad libitum, although this method is associated with obesity and an increased frequency of spontaneous tumours. It has been challenging looking for ways to limit feed consumption in group-housed rats without any setbacks to animal welfare and scientific results. The diet board, as a method of dietary restriction, was used in the present study. Diet board feeding allows group housing and should result in enhanced welfare compared with traditional methods of dietary restriction. With respect to animal model robustness and translatability of results it is important that the feeding regime does not affect diurnal rhythmicity of biological parameters. In the present study the effects of diet board feeding on diurnal rhythms of blood glucose, serum ghrelin, faecal immunoglobulin A (IgA) and faecal corticosterone were assessed. The diet board did not alter diurnal rhythms, and adds weight to the use of this method for dietary restriction which should benefit animal health and the validity of scientific results generated from the animals.

• 出版日期2018-2