A rapid and high-throughput quantum dots bioassay for monitoring of perfluorooctane sulfonate in environmental water samples

作者：Zhang Jiong; Wan Yanjian; Li Yuanyuan; Zhang Qiongfang; Xu Shunqing; Zhu Huijun; Shu Baihua^*

来源：Environmental Pollution, 2011, 159(5): 1348-1353.

DOI：10.1016/j.envpol.2011.01.011

• High-throughput
• Monitoring
• Quantum dots bioassay
• Perfluorooctane sulfonate

Currently HPLC/MS is the state of the art tool for environmental/drinking water perfluorooctane sulfonate (PFOS) monitoring. PFOS can bind to peroxisomal proliferator-activated receptor-alpha (PPAR alpha), which forms heterodimers with retinoid X receptors (RXRs) and binds to PPAR response elements. In this bioassay free PFOS in water samples competes with immobilized PFOS in ELISA plates for a given amount of PPAR alpha-RXR alpha. It can be determined indirectly by immobilizing PPAR alpha-RXR alpha-PFOS complex to another plate coated with PPAR alpha antibody and subsequent measuring the level of PPAR alpha-RXR alpha by using biotin-modified PPAR alpha-RXR alpha probes-quantum dots-streptavidin detection system. The rapid and high-throughput bioassay demonstrated a detection limit of 2.5 ng L(-1) with linear range between 2.5 ng L(-1) and 75 ng L(-1). Detection results of environmental water samples were highly consistent between the bioassay and HPLC/MS.

• 出版日期2011-5
• 单位华中科技大学