A novel exonuclease-assisted isothermal nucleic acid amplification with ultrahigh specificity mediated by full-length Bst DNA polymerase

作者：Ye, Xin; Li, Yang; Wang, Lijuan; Fang, Xueen^*; Kong, Jilie^*

来源：Chemical communications, 2018, 54(75): 10562.

DOI：10.1039/c8cc04577a

• POINT
• CARE
• DIAGNOSTICS
• PLATFORM
• SYSTEM
• PCR

Here, we introduced a novel exonuclease-assisted isothermal nucleic acid amplification (Exo-NAT) utilizing the full-length Bst DNA polymerase combined with a melting curve analysis. This method achieved an ultrahigh specificity with a good detection limit (10 2 copies) in both singleplex and multiplex detection, which was validated by detecting three diarrhea-inducing pathogens, rotavirus A, astrovirus, and adenovirus, in 42 clinical samples.

• 出版日期2018-9-25
• 单位复旦大学