Size-exclusion chromatography (HPLC-SEC) technique optimization by simplex method to estimate molecular weight distribution of agave fructans

作者：Moreno Vilet Lorena; Bostyn Stephane; Flores Montano Jose Luis; Camacho Ruiz Rosa Maria^*

来源：Food Chemistry, 2017, 237: 833-840.

DOI：10.1016/j.foodchem.2017.06.020

• Fructans
• Agave
• Size-exclusion chromatography
• Molecular weight distribution

Agave fructans are increasingly important in food industry and nutrition sciences as a potential ingredient of functional food, thus practical analysis tools to characterize them are needed. In view of the importance of the molecular weight on the functional properties of agave fructans, this study has the purpose to optimize a method to determine their molecular weight distribution by HPLC-SEC for industrial application. The optimization was carried out using a simplex method. The optimum conditions obtained were at column temperature of 61.7 degrees C using tri-distilled water without salt, adjusted pH of 5.4 and a flow rate of 0.36 mL/min. The exclusion range is from 1 to 49 of polymerization degree (180-7966 Da). This proposed method represents an accurate and fast alternative to standard methods involving multiple-detection or hydrolysis of fructans. The industrial applications of this technique might be for quality control, study of fractionation processes and determination of purity.

• 出版日期2017-12-15