Analysis of expression and comparative profile of normal placental tissue proteins and those in preeclampsia patients using proteomic approaches

作者：Jin Hong; Ma Kai dong; Hu Rong; Chen Yan; Yang Fengying; Yao Jun; Li Xiao tian^*; Yang Peng yuan

来源：Analytica Chimica Acta, 2008, 629(1-2): 158-164.

DOI：10.1016/j.aca.2008.09.015

• Preeclampsia (PE)
• Trophoblasts
• Proteomic expression profile
• Laser capture micro-dissection (LCM)
• Comparative

Preeclampsia (PE) is a complex and serious condition of pregnancy. Trophoblasts in human placenta can be separated and collected by laser capture micro-dissection (LCM). Protein in trophoblasts have been extracted and separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), finally 962 unique proteins are identified by liquid chromatography coupled to mass spectrometry (LC-MS/MS). Comparison of differential expressed proteins in normal and those in PE are investigated. Two-dimensional electrophoresis (2DE) and MS were used to identify differential expressed proteins. 13 differential expressed proteins include signal transduction protein, molecular chaperone, cell skeleton proteins are identified, in which 3 proteins are down-regulated and 10 proteins are up-regulated. They might be correlated with the cause of PE.

• 出版日期2008-11-23
• 单位复旦大学