科研之友
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摘要
Miniaturized bioanalytical systems are increasingly being used in the field of biochemical research. Immobilized enzyme microbioreactors in capillary electrophoresis (CE) have been constructed and used to fulfill the increasing demands for miniaturized bioanalytical systems. This manipulation permits low sample consumption, reduced costs, short analysis times and efficient analyses. This review provides an overview of the distinct characteristics of immobilized microbioreactors in CE. After an introduction on miniaturized microreactors, the various methods of enzyme immobilization will be discussed. The emphasis will be on two common constructions of microreactors in CE, i.e. CE-coupled microreactors, and microreactors directly integrated into the CE capillary. Such microbioreactors offer straightforward automation of reaction steps followed by separation in the same capillary.
- 出版日期2013
