Modification of TiO2 nanotube surfaces by electro-spray deposition of amoxicillin combined with PLGA for bactericidal effects at surgical implantation sites

作者：Lee Jung Hwan; Moon Seung Kyun; Kim Kwang Mahn; Kim Kyoung Nam

来源：Acta Odontologica Scandinavica, 2013, 71(1): 168-174.

DOI：10.3109/00016357.2011.654256

• implant surface coating
• electro spray deposition
• amoxicillin
• PLGA

Objective. To fabricate the antibiotic-releasing coatings on TiO2 nanotube surfaces for wide applications of implant and bone plate in medical and dental surgery, the optimal deposition time of amoxicillin/PLGA solution simultaneously performing non-toxicity and a high bactericidal effect for preventing early implant failures was found. Materials and methods. FE-SEM, ESD and FT-IR were used for confirming deposition of amoxicillin/PLGA on the TiO2 surface. Also, the elution of amoxicillin/PLGA in a TiO2 nanotube surface was measured by a UV-VIS spectrophotometer. The bactericidal effect of amoxicillin on the TiO2 nanotube surface was evaluated by using Staphylococcus aureus (S. aureus). The cytotoxicity and cell proliferation were observed by WST assay using MC3T3-E1 osteoblast cells. Results. The results indicated that the TiO2 nanotube surface controlled by electro-spray deposition time with amoxicillin/PLGA solution could provide a high bactericidal effect against S. aureus by the bactericidal effect of amoxicillin, as well as good osteoblast cell proliferation at the TiO2 nanotube surface without toxicity. Conclusions. This study used electro-spray deposition (ESD) methodology to obtain amoxicillin deposition in nanotube structures of TiO2 and found the optimal deposition time of amoxicillin/PLGA solution simultaneously performing non-toxicity and a high bactericidal effect for preventing early implant failures.

• 出版日期2013-2