A new study on the derivatization of malondialdehyde by anisidine reagent with use of UV-Vis, FT-IR, (HNMR)-H-1, (CNMR)-C-13 techniques

作者：Akbari B; Olya M E^*; Bahmaei M; Najafi F

来源：Bulgarian Chemical Communications, 2016, 48: 18-26.

• malondialdehyde
• anisidine
• colorimetric reaction
• derivatization

Derivative methods of malondialdehyde (MDA) as a biomarker of oxidative stress, often desire high temperature and long heating time. Some of which activate a color reaction such as thiobarbituric acid reactive substances (TBA), diaminonaphtalene, pentafluorophenyl hydrazine, and phenylhydrazine after placing in that harsh condition. Our attempts were to introduce a new derivative reaction between malondialdehyde and Para methoxy aniline (PMA) in order to identify MDA. The derivatization of malondialdehyde (MDA) with different concentration of para-methoxy aniline (anisidine) in an acidic medium and various time, temperature, and pH was investigated. The possible reaction of malondialdehyde and Anisidine was completed after 10 min at 75 degrees C in optimal condition. By spectrophotometric techniques, the complex formed in this process exhibits a highly specific UV spectrum with a sharp maximum at 400nm. H-1 NMR, (CNMR)-C-13, and FT-IR were confirmed the formed adduct.

• 出版日期2016