Molecular identification of Bartonella quintana infection using species-specific real-time PCR targeting transcriptional regulatory protein (bqtR) gene

作者：Liberto Maria Carla^*; Lamberti Angelo G; Marascio Nadia; Matera Giovanni; Quirino Angela; Barreca Giorgio S; Baudi Francesco; Foca Alfredo

来源：Molecular and Cellular Probes, 2011, 25(5-6): 238-242.

DOI：10.1016/j.mcp.2011.07.001

• Real-time PCR
• bqtR
• Bartonella quintana
• Bacteremia

We describe a SYBR Green l-based real-time PCR targeting Bartonella quintana transcriptional regulatory protein (bqtR) gene, recently found as invariant gene among different B. quintana strains. Melting curve analysis allowed us to discriminate between B. quintana and Bartonella henselae amplified products. We also show its usefulness in the management of a blood culture-negative patient affected by enlarged cervical lymphonodes and long-lasting fever. B. quintana DNA detection in patient whole blood samples and blood culture bottles was confirmed by sequencing and analyzing amplified products.

• 出版日期2011-12