Molecular characterization of Korean rabies virus isolates

作者：Yang Dong Kun^*; Park Young Nam; Hong Gyeong Soo; Kang Hee Kyung; Oh Yoon I; Cho Soo Dong; Song Jae Young

来源：Journal of Veterinary Science, 2011, 12(1): 57-63.

DOI：10.4142/jvs.2011.12.1.57

• characterization
• genotype I
• molecular epidemiology
• rabies virus

The nucleoprotein (N) and glycoprotein (G) of 11 Korean rabies virus (RABV) isolates collected from animals diagnosed with rabies between 2003 and 2009 were subjected to molecular and phylogenetic ana lyses. Six isolates originated from domestic animals (cattle and dogs) and five were obtained from wild free-ranging raccoon dogs. The similarities in the nucleotide sequences of the N gene among all Korean isolates ranged from 98.1 to 99 8%, while those of the G gene ranged from 97.9 to 99.3%. Based on the nucleotide analysis of the Nand G genes, the Korean RABV isolates were confirmed as genotype 1 of Lyssavirus and classified into four distinct subgroups with high similarity. Phylogenetic analysis showed that the Korean isolates were most closely related to the non-Korean NeiMeng 1025B and 857r strains, which were isolated from rabid raccoon dogs in Eastern China and Russia, respectively. These findings suggest that the Korean RABV isolates originated from a rabid raccoon dog in Northeastern Asia. Genetic analysis of the Korean RABV isolates revealed no substitutions at several antigenic sites, indicating that the isolates circulating in Korea may be pathogenic in several hosts.

• 出版日期2011-3