A new chitosan-thymine conjugate: Synthesis, characterization and biological activity

作者：Kumar Santosh; Koh Joonseok^*; Kim Hyerim; Gupta M K; Dutta P K

来源：International Journal of Biological Macromolecules, 2012, 50(3): 493-502.

DOI：10.1016/j.ijbiomac.2012.01.015

• Chitosan
• Thymine-1-yl acetic acid
• Acylation
• Conjugate
• Biomedical applications

Conjugation of chitosan with nucleobases is expected to expand its not only antimicrobial activity but also anti-cancer activity. Here, we report the synthesis of a novel chitosan-thymine conjugate by the reaction between chitosan and thymine-1-yl-acetic acid followed by acylation. The synthesized conjugate was characterized by FTIR, XRD, H-1 NMR, TGA and SEM. The microbiological screening results demonstrated the antimicrobial activity of the conjugate against bacteria viz., Escherichia coli, Staphylococcus aureus, and fungi viz.. Aspergillus niger. The chitosan-thymine conjugate also inhibited (p %26lt; 0.05) the proliferation of human liver cancer cells (HepG2) in a dose-dependent manner but had no cellular toxicity in non-cancerous mouse embryonal fibroblast cells (NIH 3T3). Thus, the chitosan-nucleobase conjugate may open a new perspective in biomedical applications.

• 出版日期2012-4-1