A barcode pitfall in Palaearctic Stagnicola specimens (Mollusca: Lymnaeidae): Incongruence of mitochondrial genes, a nuclear marker and morphology

作者：Schniebs Katrin^*; Gloeer Peter; Vinarski Maxim V; Hundsdoerfer Anna K

来源：North-Western Journal of Zoology, 2016, 12(2): 239-254.

• molecular genetics
• morphology
• Stagnicola
• hybridization

Molecular genetic analyses of the nuclear ITS-2 spacer and the two mitochondrial markers COI and a cyt-b fragment (329 bp) of the Palaearctic freshwater snail species Stagnicola corvus (Gmelin, 1791), S. montenegrinus Gloer & Pesic 2009, S. fuscus (C. Pfeiffer, 1821), and S. palustris (O. F. Muller, 1774) are inconsistent, revealing a high percentage of hybrid specimens. The results lead to different conclusions depending on the phylogenetic relationships of the species analysed. Determined as S. montenegrinus, these specimens can now be interpreted as hybrids between S. corvus and another Stagnicola species, possibly S. palustris or S. fuscus. Hybrids between S. fuscus (role of females, i.e. inheriting mitochondria) and S. palustris (role of males) can only be distinguished from "genuine" S. palustris by the mitochondrial markers COI and cyt-b, but not by morphology. The study shows that the mitochondrial COI and cyt-b genes are not nearly as suitable for phylogenetic analyses within Stagnicola as is the nuclear marker ITS-2.

• 出版日期2016-12