Hsa-mir-124-3 CpG island methylation is associated with advanced tumours and disease recurrence of patients with clear cell renal cell carcinoma

作者：Gebauer K; Peters I; Dubrowinskaja N; Hennenlotter J; Abbas M; Scherer R; Tezval H; Merseburger A S; Stenzl A; Kuczyk M A; Serth J^*

来源：British Journal of Cancer, 2013, 108(1): 131-138.

DOI：10.1038/bjc.2012.537

• renal cell carcinoma
• microRNA
• mir-124-3
• DNA methylation
• prognosis

Background: Whether methylation of the microRNA (mir)-124-3 CpG island is of relevance for the clinical course of a solid cancer and whether it shows association with clinicopathology or survival of patients with renal cell cancer (RCC) is not known as yet. %26lt;br%26gt;Methods: In a cross-sectional study, relative methylation of mir-124-3 was measured in 111 RCC samples and 77 paired normal appearing tissues using quantitative methyl-specific PCR. Results were statistically compared with tumour histology, clinicopathological parameters and disease recurrence. %26lt;br%26gt;Results: We found tumour-specific hypermethylation of mir-124-3 in samples of RCCs with clear cell histology (ccRCC) compared with paired normal appearing tissues (P%26lt;0.0001). Methylation was significantly increased in tumours with state of advanced disease (P%26lt;0.0001). Higher relative methylation was associated with worse recurrence-free survival in both univariate (hazard ratio = 9.37; P=0.0005) as well as bivariate Cox regression analyses considering age, sex, diameter of tumours and state of advanced disease, metastasis and lymph node metastases as covariates (hazard ratios = 5.9-18.2; P-values of 0.0003-0.008). %26lt;br%26gt;Conclusion: We identified mir-124-3 CpG islands (CGI) methylation as a relevant epigenetic mark for ccRCC thus underlining the need for functional studies of potentially affected signalling pathways in kidney tumour models. Methylation of mir-124-3 is suggested as an independent prognosticator for ccRCC.

• 出版日期2013-1-15