Use of a glycosylation site database to improve glycopeptide identification from complex mixtures

作者：Chalkley Robert J^*; Baker Peter R

来源：Analytical and Bioanalytical Chemistry, 2017, 409(2): 571-577.

DOI：10.1007/s00216-016-9981-2

• Glycopeptides
• Mass spectrometry
• Glycopeptide data analysis
• Protein Prospector
• N-glycosylation
• O-glycosylation

New mass spectrometry instrumentation, particularly those with electron transfer dissociation fragmentation, has made the analysis of complex glycopeptide mixtures accessible. However, software tools need to be optimized for interpretation of this type of data. Glycopeptide identification is challenging due to the number of different peptide and sugar moieties that can be combined, leading to a large number of potential compositions to consider. In this manuscript, different strategies for reducing the number of peptides and glycopeptides considered in database searching are compared. Adaptation of the software Protein Prospector to support the use of a reference modification site database doubled the number of glycopeptide IDs. The potential of this as an improved analysis strategy is discussed.

• 出版日期2017-1