AN EMERGENCY URINE BIOASSAY METHOD FOR Am-241 BY EXTRACTION CHROMATOGRAPHY AND LIQUID SCINTILLATION COUNTING

作者：Sadi Baki B^*; Li Chunsheng; Masoud Ali; Ko Raymond; Kramer Gary H

来源：Radiation Protection Dosimetry, 2010, 141(2): 134-139.

DOI：10.1093/rpd/ncq157

An emergency urine bioassay method has been developed for the determination of Am-241 in human urine samples. The method is based on extraction chromatographic separation of Am-241 from urine on a single DCA (N,N,N',N'-tetraoctyldiglycolamide) resin column followed by liquid scintillation counting of Am-241. The minimum detectable activity (MDA) for the method was 0.02 Bq. Considering the volume of urine sample (17.2 ml) used by the method; the MDA was 1.3 Bq 1(-1). Measurement accuracy (relative bias, B-r) and repeatability (relative precision, S-B) of the method were found to be -3.4 and 8.9 %, respectively, when urine samples were spiked with Am-241 (20 Bq 1(-1)). Excellent linearity (r(2) > 0.999) was established over the range of 2-200 Bq 1(-1). The method was also found to be robust (S-B=10.2 %) against matrix effects from different urine samples. Performance of the rapid bioassay method for accuracy and repeatability were evaluated against the performance criteria for radiobioassay (ANSI N13.30) and found to be in compliance. Considering the simplicity, excellent analytical figures of merit and fast sample turnaround time (<1 h), it is a very promising rapid bioassay method for supporting the medical response to an emergency where internal contamination of Am-241 is involved.

• 出版日期2010