Development of a VIGS vector based on the beta-satellite DNA associated with bhendi yellow vein mosaic virus

作者：Jeyabharathy C; Shakila H; Usha R^*

来源：Virus Research, 2015, 195: 73-78.

DOI：10.1016/j.virusres.2014.08.009

• VIGS vector
• BYVMV
• beta-satellite
• Gene silencing

Bhendi yellow vein mosaic virus (BYMV) is a monopartite begomovirus with an associated beta-satellite. beta C1 ORF encoded by the beta-satellite is the symptom determinant and a strong suppressor of post transcriptional gene silencing. To create a virus induced gene silencing vector based upon the beta-satellite associated with BYVMV the beta C1 ORF was replaced with multiple cloning sites. GFP transgene and plant endogenous genes Su, PDS, PCNA and AGO1 were cloned into beta-satellite based VIGS vector. GFP expression was silenced in the GFP expressing transgenic 16c Nicotiatta benthamiatta plants infiltrated with VIGS vector carrying GFP gene inside. N. benthamiana plants infiltrated with the VIGS vector harboring the endogenous genes Su, PDS, PCNA and AGO1 produced the phenotypic symptoms yellowing of the veins, photobleaching of the veins, stunting of the plant and upward leaf curling, respectively. Real time PCR analyses revealed a reduction in the levels of the corresponding transgene or endogenous target mRNA. The beta-satellite based VIGS vector was able to silence the target genes effectively. Hence, BYVMV beta-satellite based VIGS vector can be used in functional genomics studies.

• 出版日期2015-1-2