A comparison of two extraction methods for differentiating and characterising various Mycobacterium species and Pseudomonas aeruginosa using GC-MS metabolomics

作者：Olivier Ilse; Loots Du Toit^*

来源：African Journal of Microbiology Research, 2012, 6(13): 3159-3172.

DOI：10.5897/AJMR11.1269

• Tuberculosis
• Mycobacterium
• metabolomics
• gas chromatography mass spectrometry (GC-MS)
• biosignature

We investigated the capacity of a metabolomics research approach to characterise and differentiate between various infectious Mycobacterium species and Pseudomonas aeruginosa, and compared two extraction procedures; 1) extracting the fatty acid metabolome, and 2) extracting the total metabolome, prior to gas chromatography mass spectrometry (GC-MS) and statistical data analyses. Both extraction procedures, as part of a metabolomics study, were able to successfully differentiate between all bacterial groups investigated. The total metabolome extraction method proved the better of the two methods due to its comparative: simplicity; speed (taking less than 4 h), repeatability; extraction capacity (considering the range of compounds extracted and their relative concentrations), and; ability to extract those compounds which allow a better differentiation and characterisation of the investigated sample groups.

• 出版日期2012-4-9
• 单位西北大学