Development of transgenic rice containing a mutated p subunit of soybean beta-conglycinin for enhanced phagocytosis-stimulating activity

作者：Motoyama Takayasu; Amari Yoshiki; Tandang Silvas Mary Rose; Cabanos Cerrone; Kimura Aiko; Yoshikawa Masaaki; Takaiwa Fumio; Utsumi Shigeru; Maruyama Nobuyuki^*

来源：Peptides, 2010, 31(7): 1245-1250.

DOI：10.1016/j.peptides.2010.03.035

• Rice
• Soybean
• Bioactive peptides
• Phagocytosis-stimulating activity

Improving the nutraceutical value of rice would positively impact the health and well-being of rice consumers worldwide. Based on the three-dimensional structure of soybean beta-conglycinin, we designed a beta subunit with a strong phagocytosis-stimulating activity (m beta subunit). Here, we describe the genetic modification and production of rice seeds containing the m beta subunit as part of our aim to develop a food material that promotes human health. The m beta subunit folded correctly and was accumulated in the protein body II of rice seeds at a level similar to wild-type beta subunit. Mutant beta subunit purified from transgenic rice seeds exhibited high phagocytosis-stimulating activity, demonstrating its potential value in enhancing the nutritional value of rice.

• 出版日期2010-7