摘要

Objectives: The purpose of this study was to determine the differences in the distribution of TNF-alpha (-308) gene polymorphism among aggressive periodontitis, chronic periodontitis and periodontally healthy individuals and also to investigate whether this polymorphism is associated with gingival crevicular fluid TNF-alpha levels and periodontal disease severity. Material and methods: A total of 93 individuals were enrolled in the study including 38 aggressive periodontitis, 29 chronic periodontitis patients, and 26 healthy controls. Single nucleotide polymorphism at TNF-alpha (-308) is analyzed by PCR-RFLP method. Gingival crevicular fluid samples were analyzed for TNF-alpha, using ELISA. Results: The distribution of genotypes and allele frequencies for TNF-alpha (-308) were similar among the groups. After stratification of patients with respect to attachment level, aggressive periodontitis patients with clinical attachment level >= 4 mm was observed to have a higher frequency of TNF-alpha (-308) allele 2 compared to the chronic periodontitis patients with clinical attachment level 24 mm. No significant differences were found between the TNF-alpha levels of the different genotypes in spite of an insignificant increase in patient groups carrying TNF-alpha (-308) allele 2. Conclusion: The results of this study revealed an association between TNF-alpha (-308) allele 2 frequency and aggressive periodontitis patients with clinical attachment level >= 4 mm in the population studied.

  • 出版日期2015-4