科研之友
微信
新浪微博
Facebook
分享链接
摘要
Aims: The aim of this study was to evaluate the effectiveness of specific monoclonal IBMR3 antibodies expression in the tissues of mouse and rat liver in order to recognize specific antigen for the evaluation and comparison between Balb/c mouse and Sprague Dawley rat.
Methods: Protein extracts were obtained from four months rat and three months Balb/c mouse liver for western blot analysis. The immunoblot were consequently subjected to densitometry analysis with the aid of a bioimaging instrument. The bioimaging hardware allows the measurement of molecular weight, peak height and raw volume of the liver protein band in the rat and mouse species. This helps in the diagnosis of any pathogenic or non pathogenic antigen.
Results: The bands obtained from bioimaging were exposed to a PVDF membrane Balb/c mouse revealed two bands with molecular weight of 78.68 and 22.02 kDa. However, bioimaging results for rat liver revealed six bands with molecular weights 161.36, 66.69, 47.08, 23.23, 18.07 and 15.31 kDa. Negative protein staining for mouse and rat liver tissues were performed by using mouse IgM serotype. IgM serotype was not observed precluding any no specific antigens for the IgM negative control.
Conclusion: The bioimaging revealed different results of the expression profile in molecular weight, peak height and raw volume between the same organs or even different organs, with more bands found in rat than mouse. The results from this study suggest that the IBMR3 antigens were differentially expressed in the same animal and between mouse tissue and rat. This work will be beneficial to categorize and study the character of the IBMR3 antigen and its prospective role in tissue development.
- 出版日期2010
