Determination of Fluoxetine in the Presence of Photodegradation Products Appearing During UVA Irradiation in a Solid Phase by Chromatographic-Densitometric Method, Kinetics and Identification of Photoproducts

作者：Maslanka A; Hubicka U; Krzek J^*; Walczak M; Izworski G

来源：Acta Chromatographica, 2013, 25(3): 465-481.

DOI：10.1556/AChrom.25.2013.3.5

• fluoxetine
• determination
• photostability in solid phase
• TLC

An effect of ultraviolet A (UVA) radiation on fluoxetine stability in solid phase with and without the presence of selected metal ions was studied using the chromatographic-densitometric method. Silica gel TLC F-254 plates were used as the stationary phase and chloroform-methanol-ammonia 25% (45:4.5:0.5, v/v/v) as the mobile phase. The chromatograms were recorded densitometrically at a wavelength of lambda = 260 nm. %26lt;br%26gt;Both the concentration and kind of an ion have an effect on the photodegradation process. Kinetic studies demonstrated that fluoxetine photodegradation is the fastest in the presence of Cu(II) ions, decreases in the presence of Fe(III) and Fe(II) ions, and is the lowest in the presence of Al(III) ions. Under conditions established without the presence of metal ions, fluoxetine maintains photostability. %26lt;br%26gt;The liquid chromatography-electrospray ionization/mass spectrometry (LCESI/MS) method was applied for the identification of decomposition products noting the presence of 3-phenyl-3-hydroxypropylamine and 4-trifluoromethylphenol.

• 出版日期2013-9